Topic: Alternative testing strategies for nanomaterial safety assessment: From mechanistic studies to regulatory needs

Speaker: Ying-Jan Wang (Distinguished Professor, Institute of Environmental and Occupational Health Sciences, National Cheng Kung University)

Time: April 24, 2026 12:30-14:00

Venue: Lecture Hall 1 at College of Medicine

Abstract: This lecture focuses on recent advances in nanotoxicology and alternative testing strategies, highlighting the dual role of nanotechnology in biomedicine and toxicology and its implications for future chemical safety assessment. With the rapid development of new approach methodologies (NAMs) and adverse outcome pathways (AOPs), toxicity evaluation is shifting from traditional animal testing toward mechanism-based and integrated approaches. The speaker will present key findings on the mechanisms of nanomaterial-induced toxicity, emphasizing that autophagy dysfunction serves as an early and sensitive indicator with strong potential for alternative testing applications. Recent studies include the use of an AOP framework to elucidate chlorothalonil-induced skin sensitization and long-term aging effects, as well as the demonstration that nanoparticles can enhance radiotherapy and immunotherapy efficacy through modulation of autophagy. In addition, zinc oxide nanoparticles combined with UVB exposure have been shown to induce pyroptosis and skin damage via activation of the NLRP3 inflammasome, while in silico approaches integrating in vitro and in vivo data have been applied to elucidate the toxicity of silver nanoparticles. Beyond research, the speaker has actively promoted the 3Rs (replacement, reduction, refinement) and developed collaborative platforms for alternative testing, facilitating the application of innovative methods in the safety assessment of chemicals and nanomaterials, and fostering international scientific exchange. This lecture will provide comprehensive insights into the integration of nanotoxicology and NAMs and their future applications.