April 13, 2023 1:30-3:00 pm - Direct and Indirect Human Exposure Assessment of Environmental Contaminants - Target and Nontarget Analysis Using UPLC-MS/MS
Topic：Direct and Indirect Human Exposure Assessment of Environmental Contaminants - Target and Nontarget Analysis Using UPLC - MS/MS.
Speaker：Chen, Chia-Yang（Director of Institute of Food Safety and Health, National Taiwan University、Professor of Institute of Environmental and Occupational Health Sciences, National Taiwan University）
Time：April 13, 2023 1:30-3:00 pm
Venue：Lecture Hall Ⅳ at College of Medicine
Abstract：
Humans may be exposed to various environmental contaminants from food and drinking water ingestion. The exposure can be assessed indirectly based on the concentrations of pollutants in food and food consumption rates. Besides, biomonitoring of human specimens provides direct information on contaminant doses in humans. This presentation illustrates the exposure assessment with both biomonitoring and food survey.
The first study determined 22 perfluoroalkyl substances (PFASs), eight metabolites of phthalate esters (PAEs), six bisphenols (BPs) including BPA, and four parabens in 265 human serum and 298 urine samples with isotope-dilution techniques. After enzyme hydrolysis, serum samples were processed with Waters Ostro plates; urine samples were filtrated with Waters Sirocco plates. Extracts were analyzed with atmospheric pressure gas chromatography coupled with a tandem mass spectrometer (APGC-MS/MS) and an ultra-performance liquid chromatography/tandem mass spectrometer (UPLC-MS/MS). PFOA, PFNA, PFHxS, and PFOS were detectable in all serum samples at average concentrations of 2.2-5.1 ng/mL; 8:2 diPAP was also observed in all serum samples with a mean of 89.8 ng/mL. The means of PFBA, PFBS, and 6:2 PAP were 15-36 ng/mL in urine samples, and most PAE metabolites ranged from 23 to 104 ng/mL in urine; the positive rates of BPA, BPF, and BPS were much higher than the other three BPs with concentrations of 3.9-4.7 ng/mL; the mean concentration of methyl parabens at 419 ng/mL in urine was the highest among parabens. The levels of these contaminants are positively associated with the use of plastic tableware, consumption of seafood, and the use of lotion.
The second study processed three batches of 23 items of food with QuEChERS extraction and cleanup of PRiME HLB cartridges. Extracts were analyzed with positive and negative electrospray ionization using ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometer (UHPLC-QTOF MS). Data were acquired at All Ions MS/MS mode, and MS/MS features were matched with MS/MS libraries of 3,376 compounds. Identified compounds were prioritized with ToxPi using the information of their positive rates, signal intensity, exposure data, bioactivity, and acceptable daily intake. Further, 33 prioritized chemicals were determined in three new batches of 23 items of food, in a total of 127 samples, for confirmation using UPLC-MS/MS. Pesticides were the primary residues in vegetables, and the highest was 367 ng/g of pencycuron in bokchoy. Industrial chemicals, feed additives, and human and veterinary medicines were the major pollutants in seafood; abietic acid was detected in 7 of 32 seafood samples ranging from 23 to 89 ng/g, and ethoxyquin was detected in 40% of salmon samples in the mean of 15 ng/g. Organophosphate flame retardants were detectable in 8 of 17 grain and root crops. Di(2-ethylhexyl) adipate ranged from 0.42-39 ng/g in 23 of 31 livestock and poultry products.
The biomonitoring study determined 40 crucial endocrine-disrupting chemicals in one analytical method. The detection limits reached ppt to sub-ppb levels; the positive rates of most analytes were higher than 80% and 90% in serum and urine samples, respectively. Suspect screening and chemical prioritization were feasible with high-resolution mass spectrometry coupled with ToxPi for finding candidate analytes and facilitating the target analysis on crucial contaminants for exposure and health risk assessment.
Speaker：Chen, Chia-Yang（Director of Institute of Food Safety and Health, National Taiwan University、Professor of Institute of Environmental and Occupational Health Sciences, National Taiwan University）
Time：April 13, 2023 1:30-3:00 pm
Venue：Lecture Hall Ⅳ at College of Medicine
Abstract：
Humans may be exposed to various environmental contaminants from food and drinking water ingestion. The exposure can be assessed indirectly based on the concentrations of pollutants in food and food consumption rates. Besides, biomonitoring of human specimens provides direct information on contaminant doses in humans. This presentation illustrates the exposure assessment with both biomonitoring and food survey.
The first study determined 22 perfluoroalkyl substances (PFASs), eight metabolites of phthalate esters (PAEs), six bisphenols (BPs) including BPA, and four parabens in 265 human serum and 298 urine samples with isotope-dilution techniques. After enzyme hydrolysis, serum samples were processed with Waters Ostro plates; urine samples were filtrated with Waters Sirocco plates. Extracts were analyzed with atmospheric pressure gas chromatography coupled with a tandem mass spectrometer (APGC-MS/MS) and an ultra-performance liquid chromatography/tandem mass spectrometer (UPLC-MS/MS). PFOA, PFNA, PFHxS, and PFOS were detectable in all serum samples at average concentrations of 2.2-5.1 ng/mL; 8:2 diPAP was also observed in all serum samples with a mean of 89.8 ng/mL. The means of PFBA, PFBS, and 6:2 PAP were 15-36 ng/mL in urine samples, and most PAE metabolites ranged from 23 to 104 ng/mL in urine; the positive rates of BPA, BPF, and BPS were much higher than the other three BPs with concentrations of 3.9-4.7 ng/mL; the mean concentration of methyl parabens at 419 ng/mL in urine was the highest among parabens. The levels of these contaminants are positively associated with the use of plastic tableware, consumption of seafood, and the use of lotion.
The second study processed three batches of 23 items of food with QuEChERS extraction and cleanup of PRiME HLB cartridges. Extracts were analyzed with positive and negative electrospray ionization using ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometer (UHPLC-QTOF MS). Data were acquired at All Ions MS/MS mode, and MS/MS features were matched with MS/MS libraries of 3,376 compounds. Identified compounds were prioritized with ToxPi using the information of their positive rates, signal intensity, exposure data, bioactivity, and acceptable daily intake. Further, 33 prioritized chemicals were determined in three new batches of 23 items of food, in a total of 127 samples, for confirmation using UPLC-MS/MS. Pesticides were the primary residues in vegetables, and the highest was 367 ng/g of pencycuron in bokchoy. Industrial chemicals, feed additives, and human and veterinary medicines were the major pollutants in seafood; abietic acid was detected in 7 of 32 seafood samples ranging from 23 to 89 ng/g, and ethoxyquin was detected in 40% of salmon samples in the mean of 15 ng/g. Organophosphate flame retardants were detectable in 8 of 17 grain and root crops. Di(2-ethylhexyl) adipate ranged from 0.42-39 ng/g in 23 of 31 livestock and poultry products.
The biomonitoring study determined 40 crucial endocrine-disrupting chemicals in one analytical method. The detection limits reached ppt to sub-ppb levels; the positive rates of most analytes were higher than 80% and 90% in serum and urine samples, respectively. Suspect screening and chemical prioritization were feasible with high-resolution mass spectrometry coupled with ToxPi for finding candidate analytes and facilitating the target analysis on crucial contaminants for exposure and health risk assessment.
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